An in vitro Evaluation of Anti-inflammatory and Antimicrobial Activity of Novel Nanocomposite Material Containing Titanium Oxide, Zinc Oxide and Green Tea Extract

Abstract

Green tea is an aromatic medicinal beverage obtained from the fresh leaves and buds of plant Camellia Sinensis. It is one of the least processed type of tea which contains many bioactive components. The present study aims to assess the antimicrobial and anti-inflammatory activity of a novel nanocomposite containing TiO2, ZnO and green tea extract. In this study antimicrobial activity of the nanocomposite material against common oral pathogens like S. aureus, E. faecalis, C. albicans and S. mutans were tested by Agar well diffusion method. Anti-inflammatory effect was tested using egg albumin denaturation assay and bovine serum albumin denaturation assay. All activities yielded excellent results for TiO2, ZnO nanoparticles, and their nanocomposites. As the concentration increased, the anti-inflammatory and antimicrobial activity of the nanoparticles increased. The zone of inhibition was larger in case of S. aureus indicating that antibacterial activity was stronger against S. aureus. In the future, the novel nanocomposite developed by green synthesis is a promising material for manufacturing antimicrobial agents and bioactive coatings for therapeutic devices. The nanoparticles are incorporated into a wide range of materials to improve the effectiveness of antimicrobial therapy and reduce the toxicity. Recent researches on green tea predicts various applications based on the medicinal value to reduce the risk of many modern allopathic medicines.