Exploring the Impact of Green Tea Extract-Enhanced TiO2, ZnO Nanocomposite: Insights into Cytotoxicity via MTT Assay, Cell Morphology, and Zebrafish Embryonic Study

Abstract

The present study investigates the biological effects of a green-synthesized Titanium dioxide (TiO2) and Zinc oxide (ZnO) nanocomposite, specifically in TiO2 and ZnO synthesized using green tea extract. Titanium dioxide and zinc oxide nanocomposite was prepared using green tea extract. MTT assay was done to assess the cytotoxicity of Acidine orange staining to assess morphologic changes. Cell changes were assessed using an inverted phase contrast microscope allowing for qualitative assessment of cell morphology and viability. Zebrafish embryonic toxicology evaluation was also done to evaluate the impact of nanoparticles on embryonic development. Cytotoxicity assessments on MG-63 osteosarcoma cells reveal a concentration-dependent response, with lower concentrations (10-40 μl/ml) minimally impacting cell viability and higher concentrations (80 and 100 μl/ml) inducing significant reduction, suggesting potential for targeted cancer therapies. Morphological analysis and acridine orange staining support these findings, showing the nanocomposite's influence on cell migration and increased viability. Zebrafish embryonic toxicology study indicates concentration-dependent effects on hatching kinetics and viability rates, with a transient delay in hatching and subsequent recovery, emphasizing the dynamic impact on embryonic development. Overall, this research provides valuable insights into the nanocomposite's potential applications and raises considerations for its safety and efficacy in biological systems, calling for further exploration of its therapeutic potential and long-term consequences.