In vitro Biological Activity of (Anti-inflammatory, Anti-oxidant) Pomegranate Peel Extract Mediated Titanium Dioxide

Abstract

Pomegranate peel is considered to be a reservoir of biologically active compounds, the presence of which provides anti-inflammatory and antioxidant properties to the peel extract. Titanium is a wonder metal with broad range of application in all aspects of life.  Titanium dioxide is the most common compound of titanium. The nano particles of titanium dioxide have low toxicity, which forms the basis for its broad range of applications including cancer therapy, targeted drug delivery, photodynamic therapy, antibacterial effect, role in bone formation. In the present study green synthesis of pomegranate peel extract mediated titanium dioxide (PPETiO₂) was carried out and it’s anti-inflammatory, anti-oxidant properties were evaluated. The aim of the present study was to evaluate the  biological activity (anti-inflammatory, antioxidant) of green synthesized pomegranate peel extract  mediated titanium dioxide (PPETiO₂).  The main objective of this research was to prepare green synthesized   pomegranate peel extract mediated titanium dioxide and to analyse the biological activity namely anti-oxidant activity, and anti-inflammatory activity. The anti-inflammatory activity was evaluated using the bovine serum albumin denaturation assay (BSA), the egg albumin denaturation assay (EA) and was compared with diclofenac as standard. Antioxidant activity was measured using the 2,2 Diphenyl-1-Picryl hydraxylhydrate (DPPH) assay, the H₂O₂ assay, the ferric reducing antioxidant power (FRAP) assay. Comparison made with ascorbic acid as standard. The green synthesized pomegranate peel extract mediated titanium dioxide showed antioxidant  and anti-inflammatory activity. It was directly proportional to the concentration of the extract. Protein denaturation causes inflammation. The green synthesized   PPETiO₂ composite inhibited the protein denaturation and it was concentration dependent. The study concluded that the green synthesized PPETiO₂ has Anti-inflammatory and Anti-oxidant activity and it is concentration dependent.