In vitro Evaluation of Antiinflammatory, Antioxidant Activity of Pomegranate Peel Extract Mediated Calcium Sulphate, Titanium Dioxide Nanocomposite

Authors

  • Pradeep C. Dathan Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India Author https://orcid.org/0000-0001-6756-7616
  • Deepak Nallaswamy Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India Author https://orcid.org/0000-0001-6756-7616
  • S. Rajeshkumar Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India Author https://orcid.org/0000-0001-6756-7616
  • Arya R. Nair Department of Prosthodontics, Sri Sankara Dental College, Akathumuri, Varkala, Trivandrum 695318, India Author
  • S.S. Deepthy Department of Prosthodontics, Sri Sankara Dental College, Akathumuri, Varkala, Trivandrum 695318, India Author
  • C.V. Revathy 3Department of Prosthodontics, Sri Sankara Dental College, Akathumuri, Varkala, Trivandrum 695318, India Author
  • Shahin Ismail Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India Author
  • Suja Joseph Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India Author
  • Jose Jacob Department of Conservative Dentistry, Pushpagiri College of Dental Sciences, Thiruvalla 689107, India Author
  • Leon Jose Department of Mechanical Engineering, Indian Institute of Technology Madras, Chennai 600036, India Author

Keywords:

anti-inflammatory, antioxidant, flavanoids, punicalgin, pomegranate peel extract, calcium sulphate, titanium dioxide

Abstract

Pomegranate peel is considered to be a reservoir of biologically active compounds, the presence of which provides anti-inflammatory and antioxidant properties to the peel extract. Calcium sulphate and titanium dioxide osteoconductive materials used for bone regenaration. In the present study, a green synthesized Pomegranate peel extract mediated (PPE)  calcium sulphate,( CaSo4) and Titaniumdioxide (TiO2)   nano composite (PPE CaSO4 TiO2 NC ) was formulated, and its biological properties, specifically antioxidant and anti-inflammatory effects were evaluated. The objectives were to green synthesize Pomegranate peel extract mediated Calcium sulphate Titaniumdioxide (TiO2 nano composite and subsequently evaluate its antioxidant and anti-inflammatory activities. Anti-inflammatory activity was evaluated using, Bovine serum albumin denaturation assay (BSA), Egg albumin denaturation assay (EA) and was compared with Diclofenac sodim as standard. Antioxidant activity was measured using 2,2 Diphenyl -1- Picryl hydraxylhydrate (DPPH) assay, H2O2 assay, Ferric Reducing Antioxidant power (FRAP) assay. Comparison made with Ascorbic acid as standard. Anti-inflammatory activity was seen with all concentrations of PPE CaSO4 TiO2 NC  and in all concentrations there is  significant difference between the test material and the standard P<0.05. Significant difference was found for the antioxidant activity between PPE CaSO4 TiO2 NC and standard in all concentrations (P<0.05) The study concluded that PPE CaSO4 TiO2 NC has Anti-inflammatory and Antioxidant   activity and it is concentration dependent.

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Author Biographies

  • Pradeep C. Dathan, Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India

    Dental Surgeon, Prosthodontist

  • Deepak Nallaswamy , Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India

    Director, SIMATS

  • S. Rajeshkumar, Nanobiomedicine Lab, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India

    Professor and Chief Scientist

  • Suja Joseph, Department of Prosthodontics, Department of Pharmacology, Saveetha Dental College and Hospitals, SIMATS, Chennai 600077, India

    Research Scholar

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Published

31-07-2025

Issue

Vol. 39 No. 1 (2025)

Section

Original Research Article

License

Copyright (c) 2025 Pradeep C Dathan, Dr Deepak Nallaswamy , Dr Rajeshkumar S, Arya R Nair, Deepthy SS, C.V. Revathy, Shahin Ismail, Dr Suja Joseph, Jose Jacob, Leon Jose (Author)

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How to Cite

In vitro Evaluation of Antiinflammatory, Antioxidant Activity of Pomegranate Peel Extract Mediated Calcium Sulphate, Titanium Dioxide Nanocomposite. (2025). Trends in Biomaterials and Artificial Organs, 39(1), 15-24. https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/95

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