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Abstract

Extracellular matrix (ECM) based biomaterials plays an important role in providing suitable chemical as well as structural environment for tissue growth and regeneration. The object of this study was to compare the extent of decellularization by using ionic, non-ionic, zwitter-ionic detergents and enzymes to produce an acellular matrix from bovine omasum for therapeutic applications. Histological examination revealed that all cellular and nuclear materials were completely removed by 0.5% SDS at 48 h and basal architecture of collagen bundles showed a compact arrangement. Treatment with other detergents and enzyme at different time intervals along with different concentration does not show any significant reduction in cellularity. Examination by DNA quantification revealed that treatment with SDS (0.5%) showed significant decrease (4.30+0.14 ng/µl) in DNA content in bovine omasal laminae which showed the effectiveness of treatment for decellularization. SDS-PAGE revealed that most of the proteins were removed and leaves only the collagen which was the desired component of the extracellular matrix.

Article Details

Author Biography

Naveen Kumar*, Principal Scientist

Principal Scientist, Division of Surgery, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly (U.P.)

How to Cite
Singh, P., Sharma, A., Kumar*, N., Mahan, T., Sangeetha, P., Singh, A. K., Sharma, D., Maiti, S., Saxena, S., Shrivastava, S., & Singh, K. (2017). Process Development to Prepare an Acellular Matrix from Bovine Omasum using Biological Detergents and Enzymes. Trends in Biomaterials & Artificial Organs, 31(1), 2-8. Retrieved from https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/21

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