Robust Experimental Designs for the Development of the N-terminal proB Type Natriuretic Peptide (NT-proBNP) Assay Using Europium Chelate
Keywords:
transfer function, full factorial design, design of experimentsAbstract
The Natriuretic peptides (BNP -brain natriuretic peptide (BNP) and ProBNP are markers with high sensitivity and specificity for heart failure, regulated by intra cardiac pressure. N-terminal pro B type natriuretic peptide (NT-proBNP) is an inactive product of BNP metabolism with a long half-life. BNPs are clinically valuable tools for risk stratification in patients with heart failure. The currently available assays are costly, and we need affordable techniques to make it available to all patients in various economic strata. Lateral flow immunoassay incorporated with fluorescence can be a better tool for quantifying the level of NT-proBNP in blood samples. Developing a point-of-care quantification of NT-proBNP concentration is challenging because of its low levels in blood in normal, healthy individuals. The study aimed to reduce the assay development time and cost by developing a europium chelated-based fluorescence immunoassay using a full factorial design approach. First, the control factors were mapped, and the transfer function was generated using the full factorial design of the experiment (DOE) approach. The control factors were ranked using a Pareto chart. The main effects and interaction effects were determined and plotted. The full factorial DOE also aims to reduce product development time by arriving at an optimum design point.
Downloads
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Vanimaya, Subhash N.N., Francis Fernandez, Anugya Bhatt, Jeemon P., Sanjay G., Harikrishnan S, Manoj G (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright and Licensing
All articles published in Trends in Biomaterials and Artificial Organs are published Open Access. To ensure the widest possible dissemination of research while protecting the integrity of the original work, we utilize the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) 4.0 International License.
User Rights
Under this license, the public is free to share (copy and redistribute the material in any medium or format) under the following terms:
- Attribution: Users must give appropriate credit, provide a link to the license, and indicate if changes were made.
- Non-Commercial: Users may not use the material for commercial purposes. This includes, but is not limited to, the sale of the article or its use in promotional materials for-profit.
- No Derivatives: If a user remixes, transforms, or builds upon the material, they may not distribute the modified material.
Author Rights
Authors retain copyright of their work while granting the journal a non-exclusive license to publish. Because of the NoDerivs (ND) and Non-Commercial (NC) designations:
- Third parties (such as other researchers) must seek permission from the authors/journal to include figures, tables, or portions of the text in new works or commercial publications.
- Authors may deposit the "Version of Record" in institutional repositories immediately upon publication, provided the CC BY-NC-ND 4.0 license is clearly linked.
