Main Article Content
In spite of numerous advances in the field of reconstructive surgery, abdominal wall reconstruction remains a greater challenge even today. Various synthetic and natural materials were used in abdominal wall reconstruction. However each material has its own limitations and complications. The in vitro biocompatibility was determined to assess its performance in in vivo and further in clinical applications. Native aorta of buffalo origin was decellularized using 1% ionic biological detergent for 48 h. The acellular aortic matrix was cross-linked with Glutaraldehyde (GA), 1,4-butanediol diglycidylether (BDDGE) and 1-ethyl-3-(3-dimethyl aminopropyl carbodiimide) EDC for 12, 24, 48 and 72 h time intervals. Uncross-linked acellular aortic matrix graft was used as control. In vitro studies included enzymatic degradation, moisture content percentage, free amino group determination, fixation index, molecular weight analysis and free protein content estimation. Acellular aortic matrix graft cross-linked with 0.6% GA for 48 h and 72 h, 1% EDC and BDDGE for 24 h were found promising in in vitro studies which were later used for evaluation in in vivo studies.