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Abstract

Adipose tissue is one of the tissues used to generate mesenchymal stem cells (MSCs) and has advantages such as easy access, minimal invasiveness, as well as the possibility of harvesting a large number of cells from it. This study was conducted mainly to evaluate the growth, proliferation, and adhesion of cells to the scaffold, as well as to evaluate and compare changes in the expression of IL-1Ra, IL-10, and TNF-á genes in three conditions culture medium, control sample, scaffold containing alendronate (Aln), and scaffold without the drug. To access adipose-derived stem cells, adipose tissue was prepared and the cells were cultured in the culture medium and scaffolds after isolation. Their growth and proliferation rates were then assessed by MTT assay. Moreover, an appropriate dose of non-toxic Aln was reported. Finally, the expression of the above genes was measured by Realtime-PCR under the three conditions mentioned, and the obtained data were analyzed. All these steps were tested in 24-hour and 5-day intervals. The results indicated that the growth and proliferation of cells in the scaffold containing Aln was significantly increased compared to the empty culture medium. And the expression of the pro-inflammatory gene (TNF-a) decreased in scaffolds containing Aln drug (which has an anti-inflammatory effect) compared to scaffolds without the drug. On the other hand, the expression level of anti-inflammatory genes (IL-10 and IL-1Ra) increased in scaffolds containing drugs significantly compared to scaffolds without drugs.

Keywords

scaffold nano-hydroxyapatite alendronate inflammatory genes

Article Details

How to Cite
Ghorbani, M., Naghizadeh, A. ., Zeinalzadeh, N. ., & Roshangar, L. . (2022). Expression Analysis of Inflammatory Genes of IL-1Ra, IL-10, and TNF-a in the Adipose-Derived Stem Cells Cultured on Scaffolds Containing Alendronate. Trends in Biomaterials & Artificial Organs, 36(2), 102-106. Retrieved from https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/571