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An injectable hydrogel system is appealing for irregular contours of bone defects, as it could adapt well to the defect margin, and possess improved handling properties compared to the conventional scaffolds such as autograft, ceramic blocks, etc. Here, in this work, an injectable hydrogel system was developed using whitlockite nanoparticles (WL NPs), adipose-derived extra-cellular matrix (ECM) and oxidized alginate. The prepared ECM from adipose tissue were characterized for nucleic acid content and quantified the glycosaminoglycans and collagen. The prepared hydrogel was characterized by scanning electron microscopy and Fourier transformed infrared spectroscopy. The composite hydrogel containing WL NPs showed an enhanced storage modulus and better shear-thinning property. Endothelial cells migration studies revealed both the ECM protein and WL NPs acted as a chemoattractant for cells. In vitro tube formation assay showed that the addition of WL NPs in the hydrogel provided the angiogenic potential. Further, in vitro osteogenic differentiation exhibited an enhanced alkaline phosphatase expression for the hydrogel containing WL NPs. These studies indicate that the developed injectable hydrogel system can be a good candidate for non-load bearing bone tissue engineering applications.


injectable hydrogel decellularised ECM whitlockite nanoparticles oxidized alginate osteogenesis angiogenesis bone regeneration

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Nair, D. S., Sharath, S. S., Amirthalingam, S., Ramu, J., Iyer, S., & Jayakumar*, R. (2021). Injectable Oxidized Alginate-adipose ECM Hydrogel with Biomimetic Whitlockite Nanoparticles for Enhancing Angiogenesis and Osteogenesis. Trends in Biomaterials & Artificial Organs, 35(5), 447-453. Retrieved from

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