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The present study was undertaken to evaluate the attachment, proliferation and migration of fibroblast cells on acellular matrices and to develop bioengineered collagen matrices for reconstructive surgery. The tissues used for making 3-D scaffolds were diaphragm and pericardium of buffalo as well as skin of rat and rabbit. These tissues were subjected to different protocols for decellularisation to minimize the immunological reaction. Mouse embryonic fibroblast cells were cultured in-vitro and were seeded on decellularised tissues. These decellularised tissues as well as mouse embryonic fibroblast cells seeded tissues were subjected to histological and scanning electron microscopic examinations. Light microscopic examination of decellularised tissues revealed partial to complete decellularisation. Light microscopic as well as scanning electron microscopic examinations revealed that the mouse embryonic fibroblast cells got attached, proliferated and migrated over the acellualr matrix. The protocols for decellularisation of different tissues were standardized. The bioengineered 3-D scaffolds with mouse embryonic fibroblast cells were developed. These scaffolds can be used for reconstructive surgery.