Ficus Benjamina Leaves Derived Cellulose Scaffolds for Regeneration of Full Thickness Skin Wound in New Zealand White Rabbits

Authors

  • Prafull Kumar Singh Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Anil Kumar Gangwar Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Sangeeta Devi Khangembam Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Rajesh Kumar Verma Department of Veterinary Clinical complex (Veterinary Microbiology), College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Vipin Kumar Yadav Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Ravi Prakash Goyal Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Yogendra Singh Department of Veterinary Surgery and Radiology, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Chandra Shekher Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Acharya Narendra Deva University of Agriculture and Technology, Kumarganj, Ayodhya 224 229, India Author
  • Naveen Kumar Indian Veterinary Research Institute, Izatnagar, Bareilly Author

Keywords:

Full thickness skin defects, Decellularized Ficus benjamina leaves scaffold, Wound regeneration

Abstract

The main aim of this study was to prepare Ficus benjamina leaves derived cellulose scaffolds by treatment with 5% SDS followed by incubation in 1% TnBP solution for 48h and finaly soaked in 4% NaOCl for 36h snd these scaffolds were evaluated the for regeneration of full thickness skin wound in New Zealand white rabbits. After general anesthesia and preperation of surgical site, the full thickness wounds were created on the dorsum and remained open, reconstructed with autograft, decellularized leaf scaffold and powdered decellularized leaf scaffold in group I, II, III and IV, respectively. The warmth of the wound area was significantly (P<0.05) increased on day 7, 14 and 21 in group I, II and III animals and on day 7 and 14 in group IV animals. However, warmth of the adjoining area was significantly (P<0.05) higher on day 7, 14 and 21 in group I and II, and on day 7 and 14 in group III and IV animals. On day 21, wound completely healed in group III and IV animals. A significant (P<0.05) increase in neutrophil counts after creation of wound and transplantation of scaffold was observed on day 7 in all the animals of different groups. Histopathological observations showed complete epithelization and organized collagen formation on day 28 in group III and IV. DAPI stained and SEM analysis of tissues also showed host tissue infiltration within the scaffold. It was concluded that the decellularized Ficus benjamina leaves scaffold can be used for regeneration of full thickness skin defects.

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Published

31-01-2025

Issue

Vol. 38 No. 3 (2024)

Section

Original Research Article

License

Copyright (c) 2025 Prafull Kumar Singh, Anil Kumar Gangwar, Sangeeta Devi Khangembam, Rajesh Kumar Verma, Vipin Kumar Yadav, Ravi Prakash Goyal, Yogendra Singh, Chandra Shekher, Naveen Kumar (Author)

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How to Cite

Ficus Benjamina Leaves Derived Cellulose Scaffolds for Regeneration of Full Thickness Skin Wound in New Zealand White Rabbits. (2025). Trends in Biomaterials and Artificial Organs, 38(3), 134-146. https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/54

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