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Abstract

The bovine rumen was treated with distilled water for 2-4 h. At 4 h time interval the mucosal layer was easily peeled off. Five different protocols were used to obtain the acellular rumen matrix from the delaminated bovine rumen. The delaminated bovine rumen was subjected to ionic (SDS, 0.5% and 1%), non-ionic (triton-X100 and Tween 20, 0.5% and 1%) and zwitterionic biological detergents (Trin-BP 5% and 1%) and enzyme (trypsin, 0.5% and 1%) as biological detergents for decellularization.  The treatment with ionic detergent (1% SDS) for 24 h showed 85-90 % loss of cellularity along with presence of cellular debris. At 48 h sample showed slight increase in loss of cellularity i.e. more than 90%. At 72 h submucosal layer was completely acellular. At 96 h ECM showed thin, loosely arranged collagen fibers with very high porosity. No cellular debris was evident. In present study desired results were achieved after 96 h of treatment with 1% SDS detergent in decellularization of bovine rumen. T

Keywords

sodium dodecyl sulphate decellularization buffalo rumen

Article Details

Author Biography

Naveen Kumar, Division of Surgery, ICAR-Indian Veterinary Research Institute, Izatnagar 243122, India

Principal Scientist, Division of Surgery, Indian Veterinary Research Institute, Izatnagar-243122, Bareilly (U.P.)

How to Cite
Singh, A. K., Kumar, N., Gangwar, A., Shrivastava, S., Saxena, S., Maiti, S., Raghuvansi, P., D. Mathews, D., & Singh, K. (2021). Optimization of Protocols for Decellularization of Buffalo Rumen Matrix. Trends in Biomaterials & Artificial Organs, 35(1), 57-64. Retrieved from https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/445

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