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Abstract

Hindrance of blood brain barrier (BBB) permeability poses a major pharmacological challenge leading to inefficacy of drugs for brain pathologies. Therefore focus is shifting to targeted drug delivery with the aid of biomaterials.  Previously, we reported that Bacosides (active component of Bacopa monniera) encapsulated in targeted Lactoferrin-conjugated tri-block PEG-PLA-PCL-OH polymersomes (BAN) was more efficient in restoring scopolamine impaired memory than its natural form. Given the concept of higher therapeutic efficacy of polyherbal formulations, here we investigated the co-administering effect of BAN and coconut oil derived Lauric acid encapsulated in targeted Lactoferrin-conjugated tri-block PEG-PLA-PCL-OH polymersomes (LAN) on scopolamine challenged neurons. BAN and LAN co-administration showed marked neuroprotection and also promoted neurogenesis, neurodifferentiation, neuritogenesis and neurite elongation of mouse neuroblastoma and primary neuronal cells. Cellular changes were accompanied with upregulation of neurotrophic factor BDNF and neuronal activity dependent Arc gene expression. Further, investigations into epigenetic changes, pivotal for neural cell fate determination and differentiation revealed DNMTs (DNA (cytosine-5)-methyltransferase) and HDACs (Histone deacetylases) inhibition by BAN and LAN combined treatment. Overall our study revealed neuroprotection and neuroenhancement by BAN-LAN combination better than Rivastigmine and Rivastigmine encapsulated in Lactoferrin-conjugated tri-block PEG-PLA-PCL-OH polymersomes (RiN) possibly through epigenetic modification of neuronal genes.

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How to Cite
Goyal, K., Kumar, A., Sharma, S., Konar, A., & Koul, V. (2021). Evaluation of Bacosides and Lauric acid Nanoformulations in Regulation of Neuronal and Epigenetic Markers in Primary Culture of Mouse Cortical Neurons: A Comparative Study to Rivastigmine, a Synthetic Analog. Trends in Biomaterials & Artificial Organs, 35(1), 48-56. Retrieved from https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/423