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Abstract

Orthodontic molar bands are persistent for a longer period of time in the posterior part of the mouth and thus might be prone for microbial adhesion and colonization. The aim of the present study was to evaluate the microbial adhesion on Orthodontic stainless steel molar band material under in-vitro conditions. Microbial (Candida albicans) adhesion and growth on molar band material was assessed based on optical density (OD), biomass, metabolic activity and cell viability. Scanning electron microscopy analysis was carried out to visualize the candida adhesion. Growth OD measurements made at 600 nm upon removing the cells from the band material an OD of 0.18 compared to nil in the medium alone. Similarly, higher metabolic activity was demonstrated by the cells retrieved from band material. With regard to biomass measured, the total fresh biomass was measured as 0.90 mg/ml and the dry biomass was measured as 0.14 mg/ml.  Scanning electron microscopic analysis of band materials revealed, actively dividing candidal cells. Thus, its concluded that Candidal cells adhere and colonise liberally on the orthodontic band materials. Hence, clinicians should be aware of this and instruct patients accordingly for oral hygiene maintenance.

Keywords

Microbial adhesion Orthodontic bands Candida albicans Biofilm

Article Details

How to Cite
Harikrishnan, P. (2020). In vitro Assessment of Microbial Adhesion on Orthodontic Band Material. Trends in Biomaterials & Artificial Organs, 34(2), 44-48. Retrieved from https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/385

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