Extraction and Characterization of Fish Skin Collagen for the Fabrication of Mangiferin-Enhanced Biomaterial for Wound Healing Application
DOI:
https://doi.org/10.65795/70ddzk68Keywords:
Mahi-mahi collagen, Marine biomaterials, Mangiferin, Triple-helical structure, CytocompatibilityAbstract
Collagen is a major component of the extracellular matrix, a biocompatible and biodegradable protein which is useful in wound healing biomaterials. Mammalian collagen sources are, however, associated with potential zoonosis and immunogenicity and this has resulted in increased interest in the recovery of collagen from fish processing waste. The skin of Coryphaena hippurus (mahi-mahi) was used as an induced source of type I collagen, which was isolated by an acid-assisted extraction method which includes the following stages: alkali treatment, defatting, digestion in acetic acid, salting-out, dialysis and lyophilization. The morphology, chemical structure and conformation of the extracted collagen was determined by SEM, FTIR and CD spectroscopy, which revealed that the native triple-helical structure of collagen was retained following extraction. The antioxidant, anti-inflammatory and anti-microbial property of mangiferin made it an ideal choice to be incorporated at two different concentrations (1mg/mL and 5mg/mL) in the collagen matrix with the tricalcium phosphate (TCP) to fabricate a wound healing composite scaffold. The surface morphology, functional group composition, crystallinity, thermal stability, porosity and cell viability of the fabricated scaffolds were evaluated using SEM, FTIR, XRD, TGA/DSC, ethanol infiltration method and MTT assay, respectively. The results showed that the native structure of mahi-mahi skin collagen did not change after extraction, and mangiferin was incorporated without affecting the integrity of the scaffold. The mangiferin-loaded scaffolds showed a dose-dependent reduction in porosity as the amount of mangiferin was increased, and had good thermal stability and cytocompatibility, suggesting possible usage as a sustainable, bioactive material for wound healing applications. This research fills a significant gap in the literature by assessing the physicochemical compatibility of fish-skin collagen and mangiferin as a basis for the development of an affordable marine biomaterial for tissue engineering and regeneration-based wound care.
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Copyright (c) 2026 Varsha S., Abdul Hameed N., Saranya Srinivasan, Ashok Kumar Pandurangan, Kesavan Bhaskar Reddy, Shaheedha S.M. (Author)
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Accepted 16-07-2026
Published 30-07-2026
