Modulation of Wound Healing Markers by Multicomponent Piezoelectric Nanofibrous Dressings: A Quantitative Immunohistochemical Study
DOI:
https://doi.org/10.65795/1c0bmp97Keywords:
multicomponent dresing, excision wounds, immunohistochemistry, tissue biomarkers, wound healingAbstract
The present investigation evaluated the therapeutic performance of multicomponent nanofibrous piezoelectric wound dressings using immunohistochemical analysis of major wound-healing biomarkers. Excisional wound tissues collected from Yorkshire pig models on day 34 after injury were examined for the expression of vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA), tumor necrosis factor-α (TNF-α), and transforming growth factor-β3 (TGF-β3). Quantitative assessment of stained tissue regions was carried out with Leica LAS imaging software to compare the biological responses among the treatment groups. Among the tested formulations, WD2 and WD4 produced noticeably higher TGF-β3 expression, suggesting enhanced tissue remodeling and regenerative repair. WD4-treated wounds also showed relatively moderated TNF-α expression, indicating better control of prolonged inflammatory activity during healing. Increased α-SMA expression was observed in WD2 and WD4 groups (P ≤ 0.05), reflecting improved myofibroblast activation and wound contraction. In addition, VEGF expression gradually increased in the treated groups, with WD4 exhibiting the strongest angiogenic response. Overall, the findings indicate that WD4 creates a favorable wound-healing microenvironment characterized by controlled inflammation, enhanced angiogenesis, and accelerated tissue regeneration. These results support the potential application of multicomponent nanofibrous piezoelectric dressings as advanced biomaterials for future clinical wound-care strategies.
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S.A. Eming, P. Martin, M. Tomic-Canic, Wound repair and regeneration: mechanisms, signaling, and translation, Sci. Transl. Med. 6 (2014) 265sr6. DOI: https://doi.org/10.1126/scitranslmed.3009337
G.C. Gurtner, S. Werner, Y. Barrandon, and M.T. Longaker, Wound repair and regeneration, Nature 453 (2008) 314–321. DOI: https://doi.org/10.1038/nature07039
S. Werner, R. Grose, Regulation of wound healing by growth factors and cytokines, Physiol. Rev. 83 (2003) 835–870.
S. Barrientos, O. Stojadinovic, M.S. Golinko, H. Brem, M. Tomic-Canic, Growth factors and cytokines in wound healing, Wound Repair Regen. 16 (2008) 585–601. DOI: https://doi.org/10.1111/j.1524-475X.2008.00410.x
M. Shah, D.M. Foreman, M.W. Ferguson, Neutralization of TGF-β1 and TGF-β2 or exogenous addition of TGF-β3 to cutaneous rat wounds reduces scarring, J. Cell Sci. 108 (1995) 985–1002. DOI: https://doi.org/10.1242/jcs.108.3.985
J.W. Penn, A.O. Grobbelaar, K.J. Rolfe, The role of the TGF-β family in wound healing, burns, and scarring: a review, Int. J. Burns Trauma 2 (2012) 18–28.
R.M. Gallucci, D.K. Sloan, J.M. Heck, A.R. Murray, S.J. O’Dell, Interleukin-6 indirectly induces keratinocyte migration, J. Invest. Dermatol. 122 (2004) 764–772. DOI: https://doi.org/10.1111/j.0022-202X.2004.22323.x
S.A. Eming, T.A., Wynn, P., Martin, P. Inflammation and metabolism in tissue repair and regeneration, Science 356 (2017) 1026–1030. DOI: https://doi.org/10.1126/science.aam7928
N. Ferrara, Vascular endothelial growth factor: basic science and clinical progress, Endocr. Rev. 25 (2004) 581–611. DOI: https://doi.org/10.1210/er.2003-0027
M.G. Tonnesen, X. Feng, R.A.F. Clark, Angiogenesis in wound healing, J. Investig. Dermatol. Symp. Proc. 5 (2000) 40–46. DOI: https://doi.org/10.1046/j.1087-0024.2000.00014.x
I.A. Darby, B. Laverdet, F. Bonté, A. Desmoulière, Fibroblasts and myofibroblasts in wound healing, Clin. Cosmetic. Investigating. Dermatol. 7 (2014) 301–311. DOI: https://doi.org/10.2147/CCID.S50046
B. Hinz, Myofibroblasts, Exp. Eye Res. 142 (2016) 56–70. DOI: https://doi.org/10.1016/j.exer.2015.07.009
T.P. Sullivan, W.H. Eaglstein, S.C. Davis, P. Mertz, The pig as a model for human wound healing, Wound Repair Regen. 9 (2001) 66–76. DOI: https://doi.org/10.1046/j.1524-475x.2001.00066.x
M. Seaton, A. Hocking, N.S. Gibran, Porcine models of cutaneous wound healing, ILAR J. 56 (2015) 127–138. DOI: https://doi.org/10.1093/ilar/ilv016
J.F. Wang, M.E. Olson, C.R. Reno, J.B. Wright, D.A. Hart, The pig as a model for excisional skin wound healing, Comp. Med. 51 (2001) 341–348.
T.Y. Kuo, C.C. Huang, S.J. Shieh, Y.B. Wang, M.J. Lin, M.C. Wu, L.L.H. Huang, Skin wound healing assessment via an optimized wound array model in miniature pigs, Sci. Rep. 12(2022) 445. DOI: https://doi.org/10.1038/s41598-021-03855-y
A. Banstola, J.N.J. Reynolds, The sheep as a large animal model for the investigation and treatment of human disorders, Biology (Basel) 11 (2022) 1251. DOI: https://doi.org/10.3390/biology11091251
T. Martinello, C. Gomiero, A. Perazzi, I. Iacopetti, F. Gemignani, G.M. DeBenedictis, S. Ferro, M. Zuin, E. Martines, P. Brun, L. Maccatrozzo, K. Chiers, J.H. Spaas, M. Patruno, Allogeneic mesenchymal stem cells improve the wound healing process of sheep skin, BMC Vet. Res. 14 (2018) 202. DOI: https://doi.org/10.1186/s12917-018-1527-8
I. Ribitsch, P.M. Baptista, A. Lange-Consiglio, L. Melotti, M. Patruno, F. Jenner, E. Schnabl-Feichter, L.C. Dutton, D.J. Connolly, F.G. van Steenbeek, J. Dudhia, L.C. Penning, Large animal models in regenerative medicine and tissue engineering, Front. Bioeng. Biotechnol. 8 (2020) 972. DOI: https://doi.org/10.3389/fbioe.2020.00972
I. Bernardelli de Mattos, A.C. Tuca, F. Kukla, T. Lemarchand, D. Markovic, L.P.
Kamolz, M. Funk, A highly standardized pre-clinical porcine wound healing model, Biomedicines 12 (2024) 1697. DOI: https://doi.org/10.3390/biomedicines12081697
S. Kota, P. Dumpala, R. Sajja, R. Anantha, Phytoconstituents of Chromolaena odorata leaf extract for synthesis of CuO/Cu nanoparticles, Trends Phytochem. Res. 7 (2023) 186–206.
S. Kota, P. Dumpala, R. Sajja, R. Anantha, Heteroatom-doped carbon dots from medicinal plants as novel biomaterials, Sci. Rep. 14 (2024): 13160. DOI: https://doi.org/10.1038/s41598-024-63700-w
S. Kota, P. Dumpala, R. Sajja, R. Anantha, Functional characteristics of copper/copper oxide nanoparticles synthesized with plant extracts, Sci. Rep. 14 (2024) 30857. DOI: https://doi.org/10.1038/s41598-024-81169-5
S. Kota, R. Anantha, R. Sajja, P. Dumpala, Multicomponent nanofibrous electrospun dressings in a porcine model, 3 Biotech 15 (2025) 335. DOI: https://doi.org/10.1007/s13205-025-04460-w
M. Le, R. Naridze, J. Morrison, L.C. Biggs, L. Rhea, B.C. Schutte, V. Kaartinen, M. Dunnwald, Transforming growth factor beta 3 is required for excisional wound repair in vivo, PLoS One 7 (2012) e48040. DOI: https://doi.org/10.1371/journal.pone.0048040
I. Pastar, O. Stojadinovic, N.C.Yin, H. Ramirez, A.G. Nusbaum, A. Sawaya, S.B. Patel, L. Khalid, R.R. Isseroff, M. Tomic-Canic, Epithelialization in wound healing: a Comprehensive review, Adv. Wound Care 3 (2014) 445–464. DOI: https://doi.org/10.1089/wound.2013.0473
M. Mohiti-Asli, M. Risselada, M. Jacob, B. Pourdeyhimi, E.G. Loboa, Creation and evaluation of a new porcine model for surgical site infection, Tissue Eng. Part C Methods 23 (2017) 795–803. DOI: https://doi.org/10.1089/ten.tec.2017.0024
A.C. Tuca, I. Bernardelli de Mattos, M. Funk, D. Markovic, R. Winter, T. Lemarchand, D. Kniepeiss, S. Spendel, B. Hartmann, C. Ottoman, L.P. Kamolz, A standardized porcine model for partial-thickness wound healing, Int. J. Mol. Sci. 25 (2024) 7658. DOI: https://doi.org/10.3390/ijms25147658
S. Werner, R. Grose, Regulation of wound healing by growth factors and cytokines, Physiol. Rev. 83 (2003) 835–870. DOI: https://doi.org/10.1152/physrev.2003.83.3.835
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Accepted 13-07-2026
Published 30-07-2026
