Evaluation of Doxorubicin Loaded Gelatin Coated Iron Oxide Nanoparticles for Drug Delivery and Magnetic Hyperthermia for Anti-Cancer Treatment

Abstract

The contribution of magnetic nanoparticles to the field of cancer treatment has recently created an immense impact with various modalities. In the present work we report the fabrication of magnetic iron oxide nanoparticles (IONPs) coated with a biocompatible natural polymer gelatin, followed by cross-linking of the adsorbed model drug, doxorubicin (DOX) on the surface of the nanoparticles using a natural cross-linker genipin. The particles were characterized by evaluating the zeta potential, VSM, hyperthermia and X-ray diffraction. To investigate the morphology of the IONPs, field emission scanning electron microscopy (FE-SEM) was used. Functional and elemental group was evaluated by FTIR. The drug loading efficiency and drug release kinetics was studied, whereas the toxicity of gelatin coated IONPs was evaluated by MTT assay using MG-63 osteosarcoma cells. The nanoparticles shows good super paramagnetic properties exhibiting a potential hyperthermia effect with enhanced drug release on increase in temperature. The cell viability assay displayed that gelatin coated IONPs are non-toxic thus making it a potential candidate suitable for application in anti-cancer treatment.