Preparation of Acellular Matrix from Bovine Reticulum Using Detergents and Enzynmes

Anwarul Hasan; Naveen Kumar Sharma; Kiranjeet Singh; Ashwathy Gopinathan; V. Remya; D. B. Mondal; S. K. Maiti; A. K. Sharma; K. P. Singh

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Abstract

Extracellular matrix (ECM) based biomaterials have an established place as medical devices for wound healing and tissue regeneration. In this study acellular matrix was prepared from bovine reticulum using detergents and enzynmes. Treatment with 0.5% SDS for 48h resulted in completely acellualr matrix as evaluated by histology, SEM and DNA quantification. Treatment with enzyme does not show any significant reduction in cellularity.

Author Biography

Anwarul Hasan, Principal Scientist

Principal Scientist

Division of Surgery

Indian Veterinary Research Institute

Izatnagar-243122

Bareilly (U.P.)

How to Cite

Hasan, A., Sharma, N. K., Singh, K., Gopinathan, A., Remya, V., Mondal, D. B., Maiti, S. K., Sharma, A. K., & Singh, K. P. (2015). Preparation of Acellular Matrix from Bovine Reticulum Using Detergents and Enzynmes. Trends in Biomaterials & Artificial Organs, 29(3), 231-236. Retrieved from https://www.biomaterials.org.in/tibao/index.php/tibao/article/view/154

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Preparation of Acellular Matrix from Bovine Reticulum Using Detergents and Enzynmes

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Anwarul Hasan, Principal Scientist

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