Phytochemical Assay, Cytotoxicity and Antibacterial Activity of Selected Indian Medicinal Plants for Bioprinting Applications

Abstract

Over the decade, finding an appropriate biocompatible material has found to be one of the most challenging tasks in translational bioprinting. Developing a biomaterial from herbal plants would support the cell growth and improve the biocompatibility. Indian medicinal plants have potential antimicrobial activity and provide an alternative therapeutic source for treating uropathogens. Hence, the present study evaluated the phytochemical analysis, cytotoxic effects and antibacterial activity of selected Indian medicinal plants against uropathogenic Klebsiella pneumoniae. Twenty-four selected Indian plants were used in this study. Biochemical tests and GC-MS methods were used to analyse the phytochemical components of plant extracts. Cytotoxic potential of plant extracts was assessed using human dermal fibroblast cells. Antimicrobial susceptibility test for plant extracts was performed by agar well diffusion method and minimum inhibitory concentration assay. Among the medicinal plants, eight plants showed antibacterial activity against the K. pneumoniae. In our study wide variety of phytochemicals were identified in the plant extracts. The MTT assay yielded IC50 <250μg for the all the extracts.