Curcumin Spiroborate Ester Incorporated Hydroxyapatite–β Tricalcium Phosphate Scaffolds for Tissue Engineering Applications

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Sujina Kumari Josna Joseph Leena John Rahul S Pillai S Balachandran Annie John Annie Abraham

Abstract

BACKGROUND: Drug delivery scaffolds incorporating drugs, growth factors and cytokines are available for tissue engineering applications. On the contrary, research focusing on phytochemical incorporated tissue engineered scaffolds for stem cells and tissue regeneration/repair are limited. Curcumin, a polyphenolic phyto compound isolated from Turmeric is understood to have wide biological properties including bone mineralisation whereas pure curcumin has limited bioavailability and stability. Thus the aim of this study is to synthesise modified biostable nano curcumin incorporated Hydroxyapatite- β Tri calcium phosphate scaffolds to enable hasten tissue healing.


METHODS: Beta Tri Calcium Phosphate (β-TCP) and Hydroxy Apatite/Beta Tri Calcium Phosphate (HA-βTCP) discs were synthesised and incorporated with nanoparticles of spiroborate ester of curcumin with maleic acid (CBME) by physical adsorption. The in vitro release of curcumin in PBS were analysed spectrophotometrically. Besides, the physico-chemical characterisation of the scaffolds alone and with CBME incorporation was done by XRD, FTIR spectroscopy, Atomic Force Microscopy. Anti-oxidant and anti bacterial properties of CBME was also evaluated.


RESULTS: The CBME incorporated scaffolds turned out to be non-cytotoxic and cytocompatible depicting enhanced cellular adhesion and proliferation of L929 mouse fibroblast cells.


CONCLUSION: Thus, the findings of this study proposes biostable curcumin incorporated scaffolds for sustained drug release and as a promising candidate for bone regeneration and repair owing to its inherent osseous properties.

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