Influence of Polymer Based Scaffolds with Genipin Cross Linker for the Effective (Pore Size) Usage in Biomedical Applications

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S. Mary Stella U. Vijayalakshmi

Abstract

Human bone is composed of nano-sized organic and mineral phases. Hydroxyapatite (HAP) is the main mineral component of bone (70% of bone composition) and it is effectively used in all biomedical applications. This component has been extensively studied as a material for bone implantation, and its excellent bioactivity and osteoconductivity has been clearly established. A key component in tissue engineering for bone regeneration is the scaffold that acts as a template for cell interactions and the formation of bone-extracellular matrix that provides mechanical support to the newly formed tissue. In the present investigation, hydroxyapatite has been prepared by wet chemical method and the polymers such as PVA, PVP are to be used to prepare the scaffolds. The genipin used as cross linker to control the pore size which can enhance the cell proliferation for tissue engineering. The formations of synthetic scaffolds are characterized by FT-IR, XRD, and SEM-EDAX analysis. Invitro study of the scaffolds are analysed by SBF immersion, Hemolytic assay, biodegradation study, MTT Assay. From the study we inferred that the polymer based cross linked scaffolds demonstrated stimulatory effects to control the pore and also showing good hemolysis, control biodegradation and enhancing the cell proliferation for bone tissue engineering application.

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