Engineered Biomaterial Surfaces for Neonatal Murine Cardiomyocyte Culture Toward Understanding Cardiac Hypertrophy

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Aditi Jain Jafar Hasan Venkatraman Ravi Jaseer Mohamed Kaushik Chatterjee Nagalingam R. Sundaresan

Abstract

Cardiovascular diseases are a major cause of mortality worldwide. There is a large need to develop in vitro platforms that mimic the tissue environment to understand the molecular basis of these cardiovascular diseases. Towards that objective, specifically in the current study, we aim to develop surface engineering strategies to
support the growth of neonatal cardiomyocytes. The ECM is known to provide both topographical and biochemical cues to maintain homeostasis. Two different approaches were investigated to modify the surface for culturing neonatal cardiomyocytes, which in turn could be used for investigating cardiac hypertrophy in vitro. Firstly, we
have used keratin derived from human hair to develop a simple, efficient and cost-effective protocol for culturing the neonatal cardiomyocytes. Secondly, we also demonstrate that microscale topography can be used to drive arrangement of cells that mimic their organization in vivo and improve cardiac function.

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Original Research Articles