Bone Remodeling Around Photochemical Fortified-calcium Silicate Implants in Long Term Rabbit Femur Model

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Narasimha Raghavan G. Vignesh N. Mohana D. Sivaraman P. S. Pradeep

Abstract

Calcium silicates have been used for a long time as bone grafting and regenerative material. Advent of nano sized calcium silicate has impacted tissue engineers, leading to preparation of several composites. Delivery of growth factors plays a major role in bone tissue engineering. As already reported in our previous work, phytochemicals have been seen as alternatives to such growth factors. Calcium silicate is currently chosen as a vehicle for conveying bioactive phytochemicals. Prepared calcium silicate is added with calculated amounts of phytochemicals derived from Glycirrhiza Glabra and Butea monosperma, pressed to fabricate an implant. In vitro studies were performed in Rat mesenchymal stem cells (MSCs) which showed improved cell adhesion and viability in samples containing extracts compared to pristine calcium silicate. In vivo implantation study was done in 6 months old newzealand white rabbits. Polyfluorochrome injections were administered every week to study bone remodeling. After six months, subsequent to euthanasia, the implants were analysed histologically using ground section. The results showed excellent improvement in the healing rate and bone implant contact with phytochemical incorporated ceramics compared with controls.

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