The Healing Effect of Adipose-derived Stem Cell Conditioned Medium on Burn Wound Model

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Astheria Eryani Dewi Sukmawati Lia Damayanti Elvin Clara Angmalisang Jeanne A. Pawitan

Abstract

Adipose-derived stem cells conditioned medium (ADSC-CM) was harvested from ADSC culture, which is known to contain cytokines and
growth factors as regenerative agents. This study aim is to investigate the effect of ADSC-CM local application in promoting burn wound
healing. Male Sprague dawley rats were randomly divided into control, medium complete (MC) and conditioned medium (CM) groups. Burn
wounds were created by placing a preheated metal plate on the dorsal of thorax. The wounds were then received topical treatment according
to the groups. Skin wound tissues were collected in time course and processed by paraffin method. The wounds were evaluated based on
macroscopic observation, percentage of wound closure (digital visitrak) and microscopic observation (HE and Masson’s trichrome). ADSCCM
local applications showed to enhance wound closure by reducing the wound area compared to control (p<0.05). It also promoted the
healing process better than control group as shown in epithelialization, collagen formation and distribution of granulation tissue. The wound
closure in ADSC-CM group was prominent in day 12 and 18 after wounded whereas other groups had longer time. In conclusion, our findings
suggest the benefits of ADSC-CM in promoting burn wound healing through acceleration of wound closure and skin regeneration.

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Original Research Articles