Modification of Poly-tetrafluoroethylene Surface Properties for Biocatalytic Membrane Applications

M.S. Mohy Eldin, E. A. Soliman, A. A. El-Zatahry, D.G. Mita


β-D-Galactosidase (E from Aspergillus oryzae was immobilized onto Teflon membranes grafted using γ- irradiation technique. Various grafted copolymers with varying hydrophilic moieties were prepared using simultaneous co-polymerization to study effect of the resultant microenvironment systems wherefrom type and content of these hydrophilic moieties on the activity of catalytic membranes under both of isothermal and non-isothermal conditions. Diethylene glycol dimethacrylate (DEGDMA), as a hydrophobic monomer was co-polymerized with each of the three hydrophilic monomers; methacrylic acid (MAA), acrylamide (AAM) and 2-hydroxyethyl methacrylate (HEMA). It was found that both of grafting percentage and activity of the catalytic membranes were greatly affected by the type and content of the hydrophilic components in the graft co-polymers. Since, the incorporation of the hydrophilic components in the graft co-polymers exhibited a positive effect on the activity of the catalytic hydrophobic membranes under either of isothermal or non-isothermal conditions. The increase extent in the catalytic activity of Teflon membranes grafted with hydrophobic- hydrophilic co-polymers was ranged from 18 - 27% comparing with the catalytic activity of Teflon membrane grafted with poly-DEGDMA under isothermal conditions. Whereas, these increase extent was ranged from 127 - 188% under non-isothermal conditions. © Society for Biomaterials and Artificial Organs (India), 20090503-42

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